icon concentrators from pierce part of thermo fisher


Pierce Protein Concentrators PES, 10K MWCO, 0.5 mL - Thermo .Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for . To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume.icon concentrators from pierce part of thermo fisher,Protein Concentrators | Thermo Fisher ScientificThermo Scientific™ Pierce concentrators are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for the concentration, desalting and buffer exchange of biological samples. These products are available in four sizes (0.5 mL, 6 mL, 20 mL and 100 mL) and with a range of molecular-weight.Pierce Protein Concentrator PES, 10K MWCO, 20-100 mL - Thermo .Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for . To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume.

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Antibody Conjugation to AlphaLISA Acceptor Beads - PerkinElmer

Fisher. F60. Rotary shaker (Labquake®). Barnstead Int. 400110. LAMBDA™ 25 UV/VIS spectrophotometer. PerkinElmer, Inc. L6020060. Procedure Outline . If required, antibody solutions can be concentrated using an iCON Concentrator (Thermo. Fisher .. (compared to 25 mg/mL in the protocol described in Section 5).

AlphaLISA Assay Development Guide.pdf

Pierce. (ThermoFisher Scientific. Inc.) 28314. Streptavidin-Sepharose beads. GE Healthcare, Inc. 17-5113-01. § For lower volume additions (< 10 μL), we recommend a pipettor .. concentrated using an iCON Concentrator (Pierce # 89886), .. This section describes the method to be used to perform this selection. D1.

Primary characterization and assessment of a T. spiralis antigen for .

Oct 15, 2014 . Besides, four moults occur during this period (Kozek, 1971) with the spreading of several nematode components and a turnover of the external part antigens of .. The antigenic solution was concentrated using Pierce iCON Concentrators with a 9 kDa cut-off (Thermo Fisher Scientific, Waltham, USA) before.

Crystal structure of the TSH receptor (TSHR) bound to a blocking .

K1-70 Fab for crystallisation was concentrated to 15.5 mg/ml using iCON concentrators (20 ml, 9K MWCO; ThermoFisher Scientific, Cramlington, UK). .. quantified using the Pierce Easy-Titer Human IgG (H+L) assay kit (ThermoFisher) against a standard curve of purified K1-70 rFab as per the manufacturer's instructions.

Extraction and Clarification

Dec 14, 2017 . G-Bioscience Sample Preparation - Lysis - Fractionation - Clean-Up & Concentration - Handbook & Selection Guide (pdf) ... THERMO: The Thermo Scientific Pierce LAL Chromogenic Endotoxin Quantitation Kit is an efficient, quantitative endpoint assay for the detection of gram-negative bacterial.

Capture Compound Mass Spectrometry Sheds Light on the .

The technology exclusively probes those proteins that interact with the drug molecule that is part of the Capture Compound. .. The supernatant (soluble fraction, cytosol) from this procedure was concentrated using iCon Concentrators (Thermo Fisher Scientific, Schwerte, Germany), aliquoted, and snap frozen. The protein.

Enrichment and Analysis of Non-enzymatically Glycated Peptides .

May 9, 2007 . Micro-BCA protein assay kits and ICON™ 9K concentrators were purchased from Pierce (Rockford, IL). Sequencing-grade trypsin was purchased from Promega (Madison, WI). A Tricorn™ high performance liquid chromatography column (5 mm ×100 mm) was purchased from GE Healthcare; Glycogel™ II.

Pierce Protein Concentrators PES, 10K MWCO, 0.5 mL - Thermo .

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for . To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume.

Protein Concentrators | Thermo Fisher Scientific

Thermo Scientific™ Pierce concentrators are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for the concentration, desalting and buffer exchange of biological samples. These products are available in four sizes (0.5 mL, 6 mL, 20 mL and 100 mL) and with a range of molecular-weight.

icon concentrators from pierce part of thermo fisher,

Pierce Protein Concentrator PES, 10K MWCO, 20-100 mL - Thermo .

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for . To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume.

Thermo Scientific Pierce Protein Concentrators - Fisher Scientific

New Thermo Scientific Pierce. Protein Concentrators effectively combine simplicity, speed, capacity and recovery to yield enhanced concentration, purification and sepa- ration of proteins – even with dilute protein samples. The concentrators provide efficient concentration of biological samples containing proteins (antigens,.

Antibody Conjugation to AlphaLISA Acceptor Beads - PerkinElmer

Fisher. F60. Rotary shaker (Labquake®). Barnstead Int. 400110. LAMBDA™ 25 UV/VIS spectrophotometer. PerkinElmer, Inc. L6020060. Procedure Outline . If required, antibody solutions can be concentrated using an iCON Concentrator (Thermo. Fisher .. (compared to 25 mg/mL in the protocol described in Section 5).

Crystal structure of the TSH receptor (TSHR) bound to a blocking .

K1-70 Fab for crystallisation was concentrated to 15.5 mg/ml using iCON concentrators (20 ml, 9K MWCO; ThermoFisher Scientific, Cramlington, UK). .. quantified using the Pierce Easy-Titer Human IgG (H+L) assay kit (ThermoFisher) against a standard curve of purified K1-70 rFab as per the manufacturer's instructions.

Capture Compound Mass Spectrometry Sheds Light on the .

The technology exclusively probes those proteins that interact with the drug molecule that is part of the Capture Compound. .. The supernatant (soluble fraction, cytosol) from this procedure was concentrated using iCon Concentrators (Thermo Fisher Scientific, Schwerte, Germany), aliquoted, and snap frozen. The protein.

Trophoblastic debris modifies endothelial cell transcriptome in vitro .

Jul 29, 2016 . The mechanisms by which the fetus induces maternal physiological adaptations to pregnancy are unclear. Cellular debris, shed from the placental syncytiotrophoblast into the maternal blood and phagocytosed by maternal endothelial and immune cells, may be one of these mechanisms. Here we show that.

ERp29 Regulates ΔF508 and Wild-type Cystic Fibrosis .

Jun 17, 2011 . Proteins in the collected media were concentrated 10-fold (final volume, 0.5 ml) using an iCON Concentrator (molecular mass cutoff, 9 kDa; Thermo Scientific) according to the manufacturer's protocol. Equal amounts of cell lysate proteins were resolved by SDS-PAGE, and the corresponding volume of.

Targeted Glycoproteomic Analysis Reveals That Kappa-5 is a Major .

Mar 3, 2011 . Terminal GalNAc is a common monosaccharide in several S. mansoni life stages (6, 20, 21) including eggs (6) as part of GalNAcβ1–4GlcNAc (LDN), . Eluate fractions containing purified kappa-5 were pooled, concentrated by ICON concentrators (9K MWCO, Thermo scientific, Rockford, IL, USA), dialyzed.

Profiling of Methyltransferases and Other S-adenosyl- L - JoVE

Dec 20, 2010 . For this reason and because the natural cofactor SAM is used by further enzymes transferring other parts of the cofactor or initiating radical reactions as . Concentrate the supernatant to 14 mL by ultrafiltration (e.g. using iCON Concentrators, 7 mL/9K, from Pierce) at 2370x g and 2°C. Deplete the viscous.

icon concentrators from pierce part of thermo fisher,

Herpes Simplex Virus 1 ICP4 Forms Complexes with TFIID and .

The coverslips were washed in PBS-T eight times for 7 min each and then mounted on glass slides using Immu-mount (Thermo Scientific). The slides were analyzed and images .. (B) Copurification of TBP with TAP-ICP4 as a function of KCl concentration used to extract infected cell nuclei. Nuclear extracts and the.

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